Howtosignalprocessing
- What is brightfield scanning?
- What is bright-field microscopy used for?
- What are the advantages of brightfield?
- Which main 2 challenges can we face when using brightfield microscopy?
What is brightfield scanning?
This process involves scanning the H&E-stained slides in transillumination (brightfield) mode, creating digital images that can then be directly viewed by a pathologist on a computer screen in an environment that streamlines case management, image sharing and consultation.
What is bright-field microscopy used for?
It receives light from the light source and is responsible for the concentration of light rays on the object. Bright field microscopy is used to view fixed specimens or live cells.
What are the advantages of brightfield?
Expand all Advantages of bright-field microscopy: The optics do not change the color of the observed structures. Stains are used to make certain structures visible. Bright-field microscopy requires fewer adjustments before one can observe the specimens.
Which main 2 challenges can we face when using brightfield microscopy?
Limitations. Very low contrast of most biological samples. The practical limit to magnification with a light microscope is around 1300X. Although higher magnifications are possible, it becomes increasingly difficult to maintain image clarity as the magnification increases.
